A new study by researchers at Karolinska Institutet identifies significant sex differences in the timing of X chromosome upregulation. This finding is vital for understanding central gene-regulatory processes in early fetal development. The results are published in Nature Communications.
Balanced transcriptional dosage is essential for upholding steady-state gene expression networks in our cells, and how expression dosage modulates upon gene-copy-number variation is greatly important in biomedicine.
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Imbalanced numbers of whole chromosomes result in copy discrepancy of hundreds of genes in a single sweep and are not well tolerated by the cell. Interestingly, while autosomes are present as two active copies, the X chromosome is an exception in this context. Female cells naturally carry two X: es while male cells cope with only one. This challenges the cell as an automated system to rebalance the expression of X correctly in both females (XX) and males (XY), and failure to do so results in embryonic death.
Two central chromosome-wide mechanisms are at play to collectively correct the X dosage in our cells. X inactivation silences one X allele in females, leading to an equalized dosage between the sexes. X upregulation resolves X-to-autosomal imbalances by increasing the single active X expression in both sexes. While X-inactivation and upregulation are equally vital to attain correct dosage, the latter has, for the most part, eluded detailed investigation.
Researchers at KI have meticulously characterized these processes in parallel during mouse embryogenesis using techniques capable of quantifying the expression of each chromosome copy separately in individual cells.
Elastic process
The study, led by post-doc researcher Antonio Lentini and supervised by principal researcher Björn Reinius at the Department of Medical Biochemistry and Biophysics, identified significant sex differences in the timing of X upregulation. Surprisingly, in contrast to the prevailing model of mammalian dosage compensation, in which naïve female cells are initially subject to biallelic X upregulation followed by X inactivation correcting the X dosage, they found that the two processes occur in sync in female cells. Moreover, when blocking the X-inactivation process, X upregulation never initiates.
“We found that X upregulation is an elastic process that actively adjusts expression from one allele in response to the output of the other. It explains how expression levels can remain stable across developmental windows where the female embryo would otherwise experience severe dose imbalance”, says lead author Antonio Lentini.
A milestone in X-chromosome regulation
The results are vital for understanding central gene-regulatory processes in the early embryogenesis and provide keys to the emerging field of regenerative medicine: Reprogramming success of embryonic stem cells tends to be more challenging in females as successful dosage compensation establishment is required.
“Our study represents a milestone in X-chromosome regulation research as it conflicts with the conventional dosage compensation model held for decades, adjusting the interpretation of hundreds of studies. We provide an improved framework for understanding X-chromosome biology”, says Björn Reinius.
Source: Karolinska Institutet