New evidence from a zebrafish model of epilepsy may help resolve a debate on how epileptic seizures originate, according to Weill Cornell Medicine and New York-Presbyterian investigators. The findings may also be helpful in the discovery and development of future epilepsy drugs.
In the study, published in Brain, the researchers were able to track the activities of neurons throughout the entire brains of larval zebrafish during seizures. They showed that the attacks originated from an excess of “excitatory” over “inhibitory” brain cell activity in relatively confined brain regions and spread only when they overcame inhibitory solid activity in surrounding areas.
Seizure initiation typically occurs in regions characterized by more active excitatory cells (crimson) before spreading to regions dominated by inhibitory neurons (green). Contour lines indicate the relative timing of seizure activity in initiation (orange-red) and propagation (blue) zones. Image credit: Dr. James Niemeyer
Neurons in the brain come in two broad categories: excitatory neurons whose activity stimulates the activity of other neurons, and inhibitory neurons whose activity quiets other neurons. Some recent studies have indicated that surges in the activity of inhibitory neurons can paradoxically trigger seizures. The new findings suggest otherwise.
“What’s really nice about the zebrafish model is that we can image every brain region, and in this model, for the first time, we were able to distinguish and track the activity of both excitatory and inhibitory neurons,” said first author James Niemeyer, a postdoctoral associate in neurological surgery at Weill Cornell Medicine. “So, this is a good starting point for examining the nuanced roles of these cell types during seizures.”
Niemeyer is a member of the laboratory of co-senior author Dr. Theodore Schwartz, the David and Ursel Barnes Professor of Minimally Invasive Neurosurgery, and vice chair for clinical research at the Weill Cornell Brain and Spine Center at Weill Cornell Medicine and a neurosurgeon at NewYork-Presbyterian/Weill Cornell Medical Center. The other co-senior authors of the study are Hongtao Ma, associate professor of neuroscience research in neurological surgery, and Emre Aksay, associate professor of physiology and biophysics, both at Weill Cornell Medicine.
Epilepsy is common, afflicting roughly 50 million people worldwide at any one time. But how it originates has never been entirely clear. On the one hand, there is evidence that seizures arise from zones in the brain that favor excitatory neuron activity over the usual restraining influence of inhibitory neurons. On the other hand, several recent studies have suggested that excessive activity in inhibitory neurons may trigger seizures – some researchers have observed earlier seizure activity in these cells. This has left a gap in understanding the different roles of excitation and inhibition in seizures.
“The issue has been hard to resolve due to the challenges involved in distinguishing and tracking excitatory and inhibitory neuron activity across multiple brain regions in an awake animal,” said Schwartz, a professor of neurological surgery and neuroscience at Weill Cornell Medicine.
With the zebrafish, the researchers were able to surmount those challenges. Using unique fluorescent probes, electrical recordings, and a technique called two-photon microscopy, they simultaneously distinguished and tracked excitatory and inhibitory neurons’ activity across the brain before and during seizures induced by a standard chemical method.
They observed that seizures in this model originate in the midbrain, at sites with a heavy imbalance of excitatory over inhibitory neuronal activity. Surrounding zones were much more weighted towards inhibitory activity, and, evidently, for this reason, we're able to resist, at least briefly, the spread of seizure activity from the initiating zone.
Prior studies that pointed to inhibitory neurons as seizure triggers may, in some cases, have detected only these highly inhibitory propagation zones rather than the initiating zone, the researchers suggested.
“We did find overactive inhibitory neurons in the propagation zones, but the advantage of our approach is that we can image across all brain regions to determine where the seizure originates,” said Aksay, who is also an associate professor of computational neuroscience in computational biomedicine in the HRH Prince Alwaleed Bin Talal Bin Abdulaziz Al-Saud Institute for Computational Biomedicine at Weill Cornell Medicine.
The team plans to use its zebrafish model for epilepsy drug screening and hopes to confirm its findings in future experiments looking across multiple brain regions in a mouse model.
Source: Cornell University