Though over 8 million babies have been born through in vitro fertilization (IVF), 70 per cent of IVF implantations fail. As IVF is becoming a more common route to pregnancy in cases of infertility, there is a need for better understanding of embryonic development at this early stage.
A human blastocyst expressing GFP in some cells (green), and stained to reveal the cell membranes (orange) and nuclei (blue). Labelling with GFP allowed the authors to reveal that only one 2-cell stage blastomere contributes most of the cells to the future body. Image credit: Sergi Junyent Espinosa/Caltech
Researchers in the laboratory of Caltech's Magdalena Zernicka-Goetz, Bren Professor of Biology and Biological Engineering, study the biological processes underlying the earliest days of human development. Now, a new study from the Zernicka-Goetz lab demonstrates that when human embryos are composed of two cells, at just 1 day old, only one of these cells will create most of the fetal body cells in addition to placental cells, while the other will only create placental cells. The research changes the long-standing paradigm that the two cells contribute equally to all parts of the developing embryo at this stage, suggesting that “specification”—the phenomenon of cells having specific individual roles—happens much earlier in development than previously believed.
The findings affect how embryos intended for IVF implantation are assessed for abnormalities.
“Often, in an IVF clinic, a few placental cells from the outside of the 6-day-old embryo will be selected for a genetic diagnosis to determine if they have chromosomal abnormalities,” says Zernicka-Goetz. “Our results show that, by extrapolation, those outside cells chosen are unlikely to contribute to the fetal body. The genetic information from those cells may not be as informative as sampling the fetal cells themselves.”
A paper describing the research appears in the journal Cell.
The 1-day-old human embryo is composed of just two cells, each called a blastomere. Using embryos donated for research by IVF clinics, the team labeled blastomeres with a colored dye, then used time-lapse imaging to watch as the cells divided over the course of six days. New cells carried the same color dye as their parent cell. Through this process, the team determined that fetal body cells exclusively originated from a single blastomere, while placental cells came from both.
“In addition to being valuable information for improving IVF, our study is part of a large body of research into evolutionary processes within the body,” says postdoctoral scholar Sergi Junyent, a co-first author on the new paper. “Studying how different cell lineages populate from original cells has implications for understanding what happens after mutations, how they lead to cancer, and so on.”
Written by Lori Dajose
Source: Caltech