A protein from a heat-loving bacterium allows specific detection of SARS-CoV-2 and other viruses in a one-pot assay based on RT-LAMP technology. Such a test could simplify point-of-care diagnoses of COVID-19 and other infections.
A CRISPR-Cas system that could be used with RT-LAMP has been developed to detect target genetic material in a one-pot assay without requiring new reagents. Image credit: Anastasia Serin / KAUST 2022
RT-LAMP tests are similar to PCR tests: they detect tiny amounts of viral genetic material in a sample by amplifying it to detectable amounts. One advantage of an RT-test over PCR tests is that it is performed at a single temperature (55-65 degrees Celsius) instead of repeated cycling through different temperatures. This makes it quicker and easier to use. Its disadvantage, however, is that it is not as accurate as a PCR, as it can produce false positive results.
A fluorescent signal is produced to show that the sample tests positive for SARS-CoV-2. Image credit: Anastasia Serin / KAUST 2022
“If an additional specificity factor could be coupled with RT-LAMP to distinguish between true and false amplification, it could help to develop powerful diagnostic technologies,” said KAUST doctoral student Ahmed Mahas.
Mahas and a team of scientists working in the Laboratory for Genome Engineering and Synthetic Biology with Dr. Magdy Mahfouz, KAUST professor of bioengineering, wanted to find a CRISPR-Cas system that could be used with RT-LAMP to specifically detect target genetic material in a one-pot assay without the need for new reagents. Cas proteins are bacteria's line of defense against invading pathogens and have been used to detect amplified RNA. However, most RNA-targeting Cas13 proteins function at around 37 degrees Celsius, which is incompatible with RT-LAMP. The team wanted to find a Cas13 enzyme that targets viral RNA and is stable at the temperatures used in RT-LAMP tests.
Searching through Cas protein databases, they found a promising candidate belonging to a thermophile (heat-loving) bacteria called Herbinix hemicellulosilytica. A further search through protein databases identified another Cas protein with a similar genetic sequence in a thermophile called Thermoclostridium caenicola. Tests showed both Cas proteins were stable at relatively high temperatures, but the TccCas13a protein belonging to T. caenicola remained stable within a higher range (37-70 degrees Celsius).
Following further tests and optimizations, the team was able to use TccCas13a to develop a sensitive, robust and rapid one-pot assay called OPTIMA-dx.
The test uses RT-LAMP to amplify the number of target virus RNAs in a sample. TccCas13a then recognizes and cuts it, activating it to also cut special fluorescently tagged RNAs that are added to the mix. This produces a fluorescent signal that can be read by a mobile application also developed by the team.
OPTIMA-dx successfully detected SARS-CoV-2 in patient samples. It was also modified to detect other viruses, such as hepatitis C, or even multiple viruses at the same time. The technology has been patented, a prototype produced and clinically validated, and the team is now transitioning the product into a startup phase with the aim of mass production and commercialization.
“Finding the thermostable TccCas13a enzyme is just the start,” Mahfouz said. “We are now investigating other systems to develop biologically inspired disruptive technologies for healthcare, therapeutics and diagnostics.”
Source: KAUST